Login
Help

CIS-REGULATION

Submit your Data

  1. Region 'REG00000501'

Cis-regulatory Region

Name

Ci-tropomyosin 3 -953/82 Tbx6-2 mutated

Short name

n/a

Region ID

REG00000501

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Christopher D. Brown (2009-12-09)

Annotator

Clara Degos (2009-12-09)

Curator

Delphine Dauga (2010-02-26)

View position in WashU

Description

Transcription start site is based on the start of the first exon of the KH transcript model. This region corresponds to construct 1452 in the paper.

In this construct, the second Tbx6 regulatory motif was mutated.

Hierarchy of Regulatory Regions

Modification of Ci-tropomyosin 3 -953/82 by substitution

Regulated gene(s)

KH2012:KH.C3.661 (TPM1; TPM3; TPM4)

Construct(s) made to test this region

CONS00000472: pCi-tropomyosin 3 -953/82 Tbx6-2 mutated::LacZ

Show in situ data
Overview of functional TF binding sites in this region

4,366,6314,367,665

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 T-Box n/a TACACAACT [-112 / -104]
2 bZIP n/a TGACCTAA [-82 / -75]
3 bZIP n/a TGACGTCA [-70 / -63]
4 T-Box n/a TTATCACTT [-58 / -50]

Sequence

Aniseed Coordinates: [4,366,631 / 4,367,665] on scaffold KhC3

Transcription start site is based on the start of the first exon of the KH transcript model. Tbx6-2 Binding site mutated (TTCCTCACC) was replaced by "-".

Show sequence

Length: 1,034


TGTGGTAGACGGGCTTGCCGATTTATTGCTTTCATGGAGCGGTTTGACCGAAGGACAATT
ATCTGTTTTCACATTTTCGTGTTTCTTTTGTTTTGCAGACGGTGATTTCACGAAATAGTT
AAACAGATTCTTTGGCATTTTGGACTATAAAAAATGTATGTGTTTAAGTTGCTTCGTTCG
TAAATACAACATTATCGACATTAAAACTTAGAAATACAGTAAAACGCAAATCATAACAAG
TATGCGAATTTCATGCTGCGGCATTACTGGCGGGAAAACAGTGGGGGTTGTGCTCTTTTA
TTGACATGCTGTATAAAGTTTAACTTTGTTACAAGGAAAATCGGTTCGATTTCTTTTAAA
TCAAACGTAAATTGTATTTTATGTTTTAATAATATATTTACAAATGCTTGACACTACCAA
TTATACGTTTGAGGCTAAGCGATAATTGGCTTTAAATTAAAGAATCGCAGGTCGGCTTGT
TGCGAATTAATTAATCTACCGTACCTTCAAATATGAAGACAATTTTCAATCAAGTGTAAG
GATACACTTTCTGTATTTGAAATATGAAAAACTGTAAAACGCTTGGCGTACATGTATGCT
TATCTTCTTGAACTATTTACAGCAATCTTGCAGCAGGCTTACTACGTCATTAACACGGAG
AATGACAGCCGTCTGATTGTTCGGATGGACAACAGACAAACCAGCAAAAACAAAGCATAT
ACGAAACTATTATCATGTTTGGTTATATGTAAACTGTAGAAAACATCATAGTAGGCCTAC
GTAATATTTAAGTACAACTTGTTGGTAAAAATGTTTAAACGATTCTCCAACATCGAAAGT
GTACACAACTG--------TCTATATAGAATGACCTAATTGATGACGTCAAGCATTATCA
CTTCTACATTGGCTATATAAGAGCGAAGTTGCCTTGTTATGCATCACACACAGTCGATTC
TCACTCCACAGCAGCACATTACTTCAACTAACTTGCAAAATGGAATCAATCAAGCACAAG
ATGGAAGGTCTTAT

Reference(s)

Brown et al. (2007). Functional architecture and evolution of transcriptional elements that drive gene coexpression. Science 317(5844):1557-60

Back to Top