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CIS-REGULATION

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  1. Region 'REG00000494' > 
  2. Region 'REG00000496'

Cis-regulatory Region

Name

Ci-tropomyosin 1 -186/130 CRE2 mutated

Short name

n/a

Region ID

REG00000496

Status

In Process of Manual Curation

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Christopher D. Brown (2009-12-09)

Annotator

Clara Degos (2009-12-09)

Curator

Delphine Dauga (2010-02-26)

View position in WashU

Description

Transcription start site is based on the start of the first exon of the KH transcript model.

This region (construct 1121 in the paper) is the same that region Ci-tropomyosin 1 -186bp/130bp (construct 913 in the paper) except that the second CRE regulatory motif was mutated.

Hierarchy of Regulatory Regions

Modification of Ci-tropomyosin 1 -186/130 by substitution

Regulated gene(s)

KH2012:KH.C3.814 (TPM1; TPM3; TPM4)

Construct(s) made to test this region

CONS00000467: pCi-tropomyosin 1 -186/130 CRE2 mutated::LacZ

Show in situ data
Overview of functional TF binding sites in this region

4,231,8664,232,181

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 bHLH n/a CAGCTG [4,232,062 / 4,232,067]
2 T-Box n/a CATTACCACCTT [4,232,079 / 4,232,088]
3 bZIP n/a TGACGTCA [4,232,104 / 4,232,111]

Sequence

Aniseed Coordinates: [4,231,866 / 4,232,181] on scaffold KhC3

Transcription start site is based on the start of the first exon of the KH transcript model. CRE1 Binding site mutated (TGACGTCA) was replaced by "-".

Show sequence

Length: 315


CAAAGAAAGAATCGAAAGAAGAAATGGCGACTGTTTTTAACAACTTTCCACACCCGCAGG
TAAATCTTAATGACGTCAGACCATG-----CATTACCACCTTAGACCTGTCAACAGCTGT
CTGTCATCGAAACTTGACCTAAATCTGGAGGCTTATAAAAGGGGACGTGGGCACTCGGCA
ATACATCAGTTGCATACTACCTTCACAGTAAGTTCTTATATCTTTTCTATTGTGTTTAAA
ACCACAACCTCTAAAATGGAAGCTATCAAGAAGAAGATGACCATGTTGAAATTGGACAAA
GAGAATGCACTCGAC

Reference(s)

Brown et al. (2007). Functional architecture and evolution of transcriptional elements that drive gene coexpression. Science 317(5844):1557-60

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