- Region 'REG00000429'
Cis-regulatory Region
Name
Ci-MLC2 -438/205 2M2 mutated
Short name
n/a
Region ID
REG00000429
Status
Curated
Origin
engineered region from C. robusta formely Ciona int. type A
Type of Activity
inactive_region
Author
Kusakabe team (2008-10-16)
Annotator
Clara Degos (2010-07-12)
Curator
Delphine Dauga (2010-04-08)
The M2 motifs present in the enhancer sequence upstream the +1 of transcription of the Ci-MLC2 gene are necessary and sufficient for the expression in the muscle cells.
Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.
Coordinates in the paper : -502bp/+141bp
Modification of Ci-MLC2 -438/205 by substitution
- Ci-MLC2 -438/205 REG00000006 [ regulatory_region (extended_promoter) ]
- Ci-MLC2 -438/205 44bp mutated REG00000085 [ regulatory_region (extended_promoter) ]
- Ci-MLC2 -53/61 REG00000087 [ regulatory_region (RNApol_II_core_promoter) ]
- Ci-MLC2 -438/205 2M2 mutated REG00000429 [ inactive_region ]
- Ci-MLC2 -438/205 18bp mutated REG00000430 [ regulatory_region (extended_promoter) ]
- Ci-MLC2 -438/205 M2d mutated REG00000431 [ inactive_region ]
- Ci-MLC2 -438/205 M2p mutated REG00000432 [ regulatory_region (extended_promoter) ]
7,190,8797,191,521
Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment |
---|
Aniseed Coordinates: [7,190,879 / 7,191,521] on scaffold KhC1
This sequence was found using a blast on the JGI V1.0 with the sequence included in the publication listed bellow. The two motifs M2 had been mutated.
GCCAAACACGTAAGGAATACCCCGATGGTACATATTGGTAGCATTATGAATGCTAATACA
CAATGTTATTGTATGGTGAAAAGTGAAGAACTTTTCACCATACAATACACTTTTTAGTGT
AAAACTGTTTATACAAATTTAATTTCGAAATATTTAAAAATCATTTTCCATTCGCAAAGT
GTGTAGTATCCAAGAACGTGACTAAATAAATATGCGCTCAAAACTTTACTCTTACTTTTC
GAGGCTCATGCTGGTTGCGTTCAAGGACCAGAAGAAACACATAATCACCCGACCAAAGCG
AAACAGCTTTGTTCACAACTAATCAAATTTCTTCCACCGCCTGCCAGTCGACAGACAATT
CCGCCTGTCTATTCCCAGTCGACACGCTATCAGGTCATTATGTCTGTTATAAAAGCGCTG
GGTCGAAGTTTAACAGCATTCAGTTCTGCTTAAGCATAATCTAGCAAGCATCAAACATCC
TTCAAGACGATAAACTATTATAATGGTAAGTGTAGTTGTATGCATTGCTGGGGAAGCACC
AGTAGTAATATACCATACCGATAAAACTAGTTTAGATTGTTTAGAATTTTATGTAGGATA
AGCCTAAAACTTAATAACAACTGTCGATTTTGTCTACAGGCAG