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CIS-REGULATION

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  1. Region 'REG00000381'

Cis-regulatory Region

Name

Ci-fog -314/1 delta intron_2GATA mutated

Short name

n/a

Region ID

REG00000381

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Ute Rothbächer (2008-03-09)

Annotator

Émilie Carmona (2008-03-25)

Curator

Delphine Dauga (2010-02-24)

View position in WashU

Description

Hierarchy of Regulatory Regions

Modification of Ci-fog -314/1 intron deleted by insertion

Regulated gene(s)

KH2012:KH.C4.84 (CRX; OTX1; OTX2)

Construct(s) made to test this region

CONS00000368: pfog Ci-fog -314/1 delta intron_2GATA mutated::NLS-LacZ

Show in situ data
Overview of functional TF binding sites in this region

1,827,5071,827,708

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 ZnF_(GATA) n/a TATC [-34 / -31]
2 ZnF_(GATA) n/a GATA [-27 / -24]

Sequence

Aniseed Coordinates: [1,827,507 / 1,827,708] on scaffold KhC10

This sequence was based on JGI Version 1.0. The intron deleted (position -84/-24) was replaced by "-". The two endogenous Ci-fog GATA sites (nucleotides -303 to -286) were replaced by GATA sites from the otx neural a-element (nucleotides -1434 to -1418 of potx).

Show sequence

Length: 324


CATTATGTGTGTTATTTTTGTATA------------------------------------
------------------------TACTGTGACCACCTGCCTTGTCAGCTGAGATTAATG
TCTCTCTACTTTAGAGTTTTAATTTAAACTGCCTTCTTCTCTTGTCAATATAATATCAAG
TTTGCATGATTGACAATGGGTCGGTACAATACACGAAGCTTATTCACTATCAGAGTTTAA
CTGAACACAAGAGCTTTGGAGCTTCGGTCTTGGACCGTTTCCTGAACAATATCTAAGATA
GGAAAATTCAGGAAACGGTCCAAG

Reference(s)

Rothbächer et al. (2007). A combinatorial code of maternal GATA, Ets and beta-catenin-TCF transcription factors specifies and patterns the early ascidian ectoderm. Development 134(22):4023-32

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