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CIS-REGULATION

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  1. Region 'REG00000241'

Cis-regulatory Region

Name

Ci-sFRP1/5 6-fox sites

Short name

n/a

Region ID

REG00000241

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Clément Lamy (2007-11-13)

Annotator

Clara Degos (2010-07-23)

Curator

Delphine Dauga (2009-08-17)

View position in WashU

Description

This is a synthetic region obtained by trimerising the region containing the 2 distal-most Fox A sites. It drives expresion in all a-line ectoderm as well as in A-line vegetal cells.

Hierarchy of Regulatory Regions

Modification of Ci-sFRP1/5 -1523/-1405 by insertion

Regulated gene(s)

KH2012:KH.L171.5 (SFRP1; SFRP2; SFRP5)

Construct(s) made to test this region

CONS00000233: pCi-SFRP1/5 6-Fox pbra::NLS-LacZ

Show in situ data
Overview of functional TF binding sites in this region

34,01734,044

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 Fox n/a AAACA [-1,518 / -1,514]
2 Fox n/a TGTTT [-1,506 / -1,502]
3 Fox n/a AAACA [-1,491 / -1,487]
4 Fox n/a TGTTT [-1,479 / -1,475]
5 Fox n/a AAACA [-1,464 / -1,460]
6 Fox n/a TGTTT [-1,452 / -1,448]

Sequence

Aniseed Coordinates: [34,017 / 34,044] on scaffold KhL171

Artificial sequence containing 6 Fox binding sites (a trimer of region Fox1-Fox2 sites).

Show sequence

Length: 81


TCAGCAAACAAACGACTTGTTTACTTTTCAGCAAACAAACGACTTGTTTACTTTTCAGCA
AACAAACGACTTGTTTACTTT

Reference(s)

Lamy et al. (2006). Ci-FoxA-a is the earliest zygotic determinant of the ascidian anterior ectoderm and directly activates Ci-sFRP1/5. Development 133(15):2835-44

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