Login
Help

CIS-REGULATION

Submit your Data

  1. Region 'REG00000233'

Cis-regulatory Region

Name

Ci-sFRP1/5 -1523/-1405 Fox3 mutated

Short name

n/a

Region ID

REG00000233

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Clément Lamy (2007-11-12)

Annotator

Patrick Lemaire (2007-11-12)

Curator

Delphine Dauga (2010-02-26)

View position in WashU

Description

Region from -1523 to -1405 is the minimal enhancer driving the of the earliest Ci-sFRP1/5 expression in all a-line from the 64-cell stage. This region contains essential Fox binding sites, and is a mutated derivative of sFRP -118bp (Lamy et al, 2006), in which the proximal most Fox site (TGTTT) was mutated into TGCTT. This mutation leads to a loss of activity in the a-line ectoderm.

Hierarchy of Regulatory Regions

Modification of Ci-sFRP1/5 -1523/-1405 by substitution

Regulated gene(s)

KH2012:KH.L171.5 (SFRP1; SFRP2; SFRP5)

Construct(s) made to test this region

CONS00000232: pCi-SFRP1/5 -1523/-1405 Fox3 mutated pFOG::NLS-LacZ

Show in situ data
Overview of functional TF binding sites in this region

33,92434,041

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 Fox n/a TGTTT [-1,509 / -1,505]
2 Fox n/a AAACA [33,928 / 33,932]

Sequence

Aniseed Coordinates: [33,924 / 34,041] on scaffold KhL171

JGI v1.0 version of the sequence. the proximal most Fox site (Fox 3) was mutated from TGTTT to TGcTT.

Show sequence

Length: 118


GCAAACAAACGACTTGTTTACTTTTTTTCTTGTCCCGTTAACAGATTAGGAGTTGGTGCC
AGAGGATTGATTTCATTGTAATCTGTTGGTGTTGTGGCTCCAATTTAAGCAAACGTGC

Reference(s)

Lamy et al. (2006). Ci-FoxA-a is the earliest zygotic determinant of the ascidian anterior ectoderm and directly activates Ci-sFRP1/5. Development 133(15):2835-44

Back to Top