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CIS-REGULATION

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  1. Region 'REG00000228'

Cis-regulatory Region

Name

Ci-sFRP1/5 -1523/-1405 Fox1-2 mutated

Short name

n/a

Region ID

REG00000228

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Clément Lamy (2007-11-12)

Annotator

Patrick Lemaire (2007-11-12)

Curator

Delphine Dauga (2010-02-26)

View position in WashU

Description

Region from -1523 to -1405 is the minimal enhancer driving the of the earliest Ci-sFRP1/5 expression in all a-line from the 64-cell stage.
This region contains essential Fox binding sites, and is a mutated derivative of sFRP -118bp (Lamy et al, 2006), in which the distal most 2 Fox sites (TGTTT) are mutated into TGCTT. These mutations lead to a stiking reduction in the pan anterior a-line activity of the region and to a slight increase in the neural b-line activity. residual a-line and b-line activities are MEK dependent and therefore distinct from endogenous regulation.

Hierarchy of Regulatory Regions

Modification of Ci-sFRP1/5 -1523/-1405 by substitution

Regulated gene(s)

KH2012:KH.L171.5 (SFRP1; SFRP2; SFRP5)

Construct(s) made to test this region

CONS00000223: pCi-SFRP1/5 -1523/-1405 Fox1-2 mutated pFOG::NLS-LacZ

Show in situ data
Overview of functional TF binding sites in this region

33,92434,041

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 Fox n/a AAACA [34,023 / 34,027]

Sequence

Aniseed Coordinates: [33,924 / 34,041] on scaffold KhL171

JGI v1.0 version. The 2 mutations are in small caps.

Show sequence

Length: 118


GCAAGCAAACGACTTGCTTACTTTTTTTCTTGTCCCGTTAACAGATTAGGAGTTGGTGCC
AGAGGATTGATTTCATTGTAATCTGTTGGTGTTGTGGCTCCAATTTAAACAAACGTGC

Reference(s)

Lamy et al. (2006). Ci-FoxA-a is the earliest zygotic determinant of the ascidian anterior ectoderm and directly activates Ci-sFRP1/5. Development 133(15):2835-44

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