Description

- when placed upstream of the pfog basal promoter, activity detected in the neural precursors b6.5 at 110 cell stage.


- Coordinates on the V1.0 ciona genome: scaffold_490:18415-18584 (s490:18415)

Hierarchy of Regulatory Regions

Modification of Ci-Nodal -2260/-1732 by deletion

Regulated gene(s)

KH2012:KH.L106.16 (BMP10; GDF2; NODAL)

Construct(s) made to test this region

CONS00000222: Nodal -1918/-1771 pFOG::LacZ

CONS00001516: p.Ci-Nodal -1918/-1771>H2B::mCherry

Overview of functional TF binding sites in this region

33,38933,535

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment
1	Fox	n/a	AAACA	[-1,894 / -1,890]
2	ZnF_(GATA)	n/a	GATA	[-1,887 / -1,884]
3	ZnF_(GATA)	n/a	TATC	[-1,868 / -1,865]
4	Ets	n/a	GGAA	[-1,854 / -1,851]
5	Fox	n/a	AAACA	[-1,852 / -1,848]
6	ZnF_(GATA)	n/a	TATC	[-1,827 / -1,824]
7	Ets	n/a	GGAA	[-1,814 / -1,811]
8	ZnF_(GATA)	n/a	TATC	[-1,783 / -1,780]

Sequence

Aniseed Coordinates: [33,389 / 33,535] on scaffold KhL106

Sequence from JGI V1.0

Reference(s)

Stolfi et al. (2015). Migratory neuronal progenitors arise from the neural plate borders in tunicates. Nature 527(7578):371-4

Khoueiry et al. (2010). A cis-regulatory signature in ascidians and flies, independent of transcription factor binding sites. Curr. Biol. 20(9):792-802