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CIS-REGULATION

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  1. Region 'REG00000220'

Cis-regulatory Region

Name

Ci-sFRP1/5 -1601/-1475

Short name

n/a

Region ID

REG00000220

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Clément Lamy (2007-11-10)

Annotator

Patrick Lemaire (2007-11-10)

Curator

Delphine Dauga (2010-02-26)

View position in WashU

Description

Region from -1601 to -1475 is a weak enhancer driving expression in neural a-line from the 110-cell stage.
This region contains some essential Fox binding sites, and is named sFRP-126 (Lamy et al, 2006).

Hierarchy of Regulatory Regions

Modification of Ci-sFRP1/5 -1601/-1453 by deletion

Regulated gene(s)

KH2012:KH.L171.5 (SFRP1; SFRP2; SFRP5)

Construct(s) made to test this region

CONS00000215: pCi-SFRP1/5 -1601/-1475 pFOG::NLS-LacZ

Show in situ data
Overview of functional TF binding sites in this region

33,99434,119

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 Fox n/a TGTTT [-1,509 / -1,505]
2 Fox n/a AAACA [34,036 / 34,040]
3 Fox n/a AAACA [34,076 / 34,080]

Sequence

Aniseed Coordinates: [33,994 / 34,119] on scaffold KhL171

JGI v1.0 version

Show sequence

Length: 126


CGGGTTCTCATAGAATCTACTAATTACATAAAAGTCCAACAAAAACAAGTTTAATCGATT
GACTCAAACTCGTTATCAGCAAACAAACGACTTGTTTACTTTTTTTCTTGTCCCGTTAAC
AGATTA

Reference(s)

Lamy et al. (2006). Ci-FoxA-a is the earliest zygotic determinant of the ascidian anterior ectoderm and directly activates Ci-sFRP1/5. Development 133(15):2835-44

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