Description

This sequence is located close to the prickle-like gene but may not be involved in its regulation. No activity detected at 110 cell-stage when placed in front of the p-fog basl promoter driving LacZ.


Coordinates are base on KH transcript model.

Hierarchy of Regulatory Regions

Regulated gene(s)

KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3)

Construct(s) made to test this region

CONS00000210: Prickle Like 10773/10946 pFOG::LacZ

Overview of functional TF binding sites in this region

1,330,3961,330,568

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment
1	Ets	n/a	GTTCCC	[1,330,402 / 1,330,407]
2	Ets	n/a	ATTCCT	[1,330,408 / 1,330,413]
3	ZnF_(GATA)	n/a	TGATAG	[1,330,415 / 1,330,420]
4	Ets	n/a	TTTCCG	[1,330,443 / 1,330,448]
5	ZnF_(GATA)	n/a	AGATAA	[1,330,468 / 1,330,473]
6	Ets	n/a	GGGAAA	[1,330,474 / 1,330,479]
7	Ets	n/a	CTTCCA	[1,330,483 / 1,330,488]
8	Ets	n/a	AGGAAA	[1,330,496 / 1,330,501]
9	ZnF_(GATA)	n/a	CTATCT	[1,330,522 / 1,330,527]

Sequence

Aniseed Coordinates: [1,330,396 / 1,330,568] on scaffold KhC8

Sequence from JGI V1.0
PCR on genomic DNA using the following primers:
FWD: GATC designed primer
REV: GATC designed primer

Reference(s)

Khoueiry et al. (2010). A cis-regulatory signature in ascidians and flies, independent of transcription factor binding sites. Curr. Biol. 20(9):792-802