- Region 'REG00000210'
Cis-regulatory Region
Name
Ci-Mesp -110/39 T-boxB mutatedT
Short name
n/a
Region ID
REG00000210
Status
Curated
Origin
engineered region from C. robusta formely Ciona int. type A
Type of Activity
regulatory_region
(enhancer)
Author
Brad Davidson (2007-11-01)
Annotator
Patrick Lemaire (2007-11-01)
Curator
Delphine Dauga (2010-02-26)
The minimal cardiac enhancer for the ciona Mesp gene demonstrated direct activation by the T-box transcription factor Tbx6c. In this mutated enhancer, the T-box site 1 is point mutated in a neutral way that does not prevent binding of tbx6c.
JGI transcript model was taken as reference for the region s coordinates (same in the paper).
Modification of Ci-Mesp -110/39 by substitution
- Ci-Mesp -150bp/39bp REG00000643 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -138/39 REG00000211 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -150bp/39bp Lhx3-1 mutated REG00000644 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -150bp/39bp Lhx3-2 mutated REG00000645 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -150bp/39bp Lhx3-3 mutated REG00000646 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -150bp/39bp Lhx3-4/5 mutated REG00000647 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -150bp/39bp Lhx3-6 mutated REG00000648 [ regulatory_region (extended_promoter) ]
3,537,1593,537,296
| Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment | |
|---|---|---|---|---|---|
| 1 | T-Box | n/a | AGGTGTGAAC | [-110 / -101] | |
| 2 | T-Box | n/a | CGGCGATAA | [-82 / -74] | |
| 3 | T-Box | n/a | TAGACACCT | [-63 / -55] |
Aniseed Coordinates: [3,537,159 / 3,537,296] on scaffold KhC3
The sequence was retrieved from the JGI v1.0 assembly using the sequence found in the paper. T-box site B: AGGCGATAAC is mutated into AGGTGTGAAC, which is a functional T-box site.
Show sequence
Length: 148
AGGTGTGAACGACTTTGCCCGTTTCATGCGGCGATAAACGAACTAATTAGACACCTCCTA
CAGATATAATGGTAATTCAGAATCGTGTGGTTATGTAATTCACAAAAACATTTTAACAAA
ACAGGTTGATTTGAAACTTGTATTATGG
