- Region 'REG00000208'
Cis-regulatory Region
Name
Ci-Mesp -110/39 T-BoxB mutated1
Short name
n/a
Region ID
REG00000208
Status
Curated
Origin
engineered region from C. robusta formely Ciona int. type A
Type of Activity
inactive_region
Author
Brad Davidson (2007-11-01)
Annotator
Patrick Lemaire (2007-11-01)
Curator
Delphine Dauga (2010-02-26)
The minimal cardiac enhancer for the ciona Mesp gene demonstrates direct activation by the T-box transcription factor Tbx6c. Inactivation of site B by mutation kills the activity of this region.
JGI transcript model was taken as reference for the region s coordinates (same in the paper).
Modification of Ci-Mesp -110/39 by substitution
- Ci-Mesp -150bp/39bp REG00000643 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -138/39 REG00000211 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -150bp/39bp Lhx3-1 mutated REG00000644 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -150bp/39bp Lhx3-2 mutated REG00000645 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -150bp/39bp Lhx3-3 mutated REG00000646 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -150bp/39bp Lhx3-4/5 mutated REG00000647 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -150bp/39bp Lhx3-6 mutated REG00000648 [ regulatory_region (extended_promoter) ]
3,537,1593,537,301
| Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment | |
|---|---|---|---|---|---|
| 1 | T-Box | n/a | CGGCGATAA | [-82 / -74] | |
| 2 | T-Box | n/a | TAGACACCT | [-63 / -55] |
Aniseed Coordinates: [3,537,159 / 3,537,301] on scaffold KhC3
The sequence was retrieved from the JGI v1.0 assembly using the sequence found in the paper. T-box site B, AGGCTA, was inactivated by mutation to AGTCAA.
Show sequence
Length: 148
AGTCAATAATGACTTTGCCCGTTTCATGCGGCGATAAACGAACTAATTAGACACCTCCTA
CAGATATAATGGTAATTCAGAATCGTGTGGTTATGTAATTCACAAAAACATTTTAACAAA
ACAGGTTGATTTGAAACTTGTATTATGG
