Aniseed - Cis-regulation

Region 'REG00000205'

Cis-regulatory Region

Name

FoxF -1072/-777 EtsA,B mutated

Short name

n/a

Region ID

REG00000205

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Jeni Beh (2007-10-26)

Annotator

Patrick Lemaire (2007-10-26)

Curator

Delphine Dauga (2010-03-31)

View position in WashU

Description

Transcription start site is based on the start of the first exon of the KH transcript model.

Coordinates in the paper : -1135/-840.

Hierarchy of Regulatory Regions

Modification of FoxF -1072/-777 by substitution

FoxF -2989/64 REG00000192 [ regulatory_region (extended_promoter) ]

Regulated gene(s)

KH2012:KH.C3.170 (FOXF1; FOXF2; FOXL1)

Construct(s) made to test this region

CONS00000200: pFoxF -1072/-777 EtsA,B mutated pfkh::LacZ

Show in situ data

Overview of functional TF binding sites in this region

3,598,2493,598,543

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment
1	bHLH	n/a	CACTTG	[-914 / -909]
2	Ets	n/a	TTTCCG	[-797 / -792]

Sequence

Aniseed Coordinates: [3,598,249 / 3,598,543] on scaffold KhC3

The EtsA (AGGATT) and EtsB (AGGATG) were mutated into ATAGTT and ATAGTG, respectively. The sequence was found in the paper.

Show sequence

Length: 295

GCGTTGTATCTTTCGTTAAGTGCTGGCGTCGAGCAGGGCTAACCTCATTTCGATTTGACG
ATTCGGGTAAAACCGAGCGATGGACAGGACCTGGAAATGGGAAAGGCTTACTAGAATCAG
GGTCGAACGAGATAAGAAGATCATAGTTCGCAAATTAGCACTTGGCTTTTTTACGTAGAA
ACATATAGTGTCCGCTGAGCCCCTTATATATACGTAGTACGAATCACCGGCCTGGCGTTG
TATAAGCGAGACCAGCGTGGACGAAAAGGTAATTGTTTCCGAATGGATCGCGTTC

Reference(s)

Beh et al. (2007). FoxF is essential for FGF-induced migration of heart progenitor cells in the ascidian Ciona intestinalis. Development 134(18):3297-305