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CIS-REGULATION

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  1. Clone 'cien203265' > 
  2. Region 'REG00001017' > 
  3. Molecular Tool 'Ci-FoxD/Ci-lefty' > 
  4. Molecular Tool 'pGsx>OC::Vp16 (Vassalli 2021)' > 
  5. Region 'REG00000202'

Cis-regulatory Region

Name

FoxF -1072/-777 EtsA mutated

Short name

n/a

Region ID

REG00000202

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Jeni Beh (2007-10-26)

Annotator

Patrick Lemaire (2007-10-26)

Curator

Delphine Dauga (2010-03-31)

View position in WashU

Description

Transcription start site is based on the start of the first exon of the KH transcript model.

Coordinates in the paper : -1135/-840.

Hierarchy of Regulatory Regions

Modification of FoxF -1072/-777 by substitution

Regulated gene(s)

KH2012:KH.C3.170 (FOXF1; FOXF2; FOXL1)

Construct(s) made to test this region

CONS00000197: pFoxF -1072/-777 EtsA mutated pfkh::LacZ

Show in situ data
Overview of functional TF binding sites in this region

3,598,2493,598,543

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 bHLH n/a CACTTG [-914 / -909]
2 Ets n/a AGGATG [-888 / -883]
3 Ets n/a TTTCCG [-797 / -792]

Sequence

Aniseed Coordinates: [3,598,249 / 3,598,543] on scaffold KhC3

The EtsA site AGGATT, was mutated into ATAGTT. The sequence was found in the paper.

Show sequence

Length: 295


GCGTTGTATCTTTCGTTAAGTGCTGGCGTCGAGCAGGGCTAACCTCATTTCGATTTGACG
ATTCGGGTAAAACCGAGCGATGGACAGGACCTGGAAATGGGAAAGGCTTACTAGAATCAG
GGTCGAACGAGATAAGAAGATCATAGTTCGCAAATTAGCACTTGGCTTTTTTACGTAGAA
ACATAGGATGTCCGCTGAGCCCCTTATATATACGTAGTACGAATCACCGGCCTGGCGTTG
TATAAGCGAGACCAGCGTGGACGAAAAGGTAATTGTTTCCGAATGGATCGCGTTC

Reference(s)

Beh et al. (2007). FoxF is essential for FGF-induced migration of heart progenitor cells in the ascidian Ciona intestinalis. Development 134(18):3297-305

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