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CIS-REGULATION

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  1. Region 'REG00000524'
  2. Region 'REG00000187'

Cis-regulatory Region

Name

Ci-Gai1 -4208/-1

Short name

n/a

Region ID

REG00000187

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Reiko Yoshida (2007-08-02)

Annotator

Delphine Dauga (2010-01-11)

Curator

Delphine Dauga (2010-02-24)


Description

Region from -4208bp to the start codon allows expression in the
nervous systems (in the adhesive organ, brain vesicle, visceral ganglion, and dorsocaudal epidermal neurons on the dorsal midline
of the tail) and in the mesenchyme. No GFP expression was observed in tissues other than the nervous system and mesenchyme cells.
The region between -3176 and -3090 indicates a highly conserved region, Gai1-CNR.

Construct(s) made to test this region

CONS00000182: pGai1 -4208/-1::EGFP

Overview of functional TF binding sites in this region

4,935,4134,939,619

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [4,935,413 / 4,939,619] on scaffold KhC1

This sequence was found using JGI V1.0. Relative coordinates were taken in the articles (+1 refers to the first exon of JGI gene model).

Show sequence

Length: 4,207

GTGACCTTTCATTTGCTGCTTTATCGTCTGTGGAGACTGTGCAACCCATGATTCCGGCTG
TGCTTGTTCGTATAAACACCTAATAGCAGATTAAAAGCTGTTTTACGCCAAGCGCCCTTA
ATGTTACACCTGCAAGCATATATGGTGCAAGGTATACAGATTAATTGAAAGTCCTTGTAT
TTTCAAACAGAGATAGCTCGTATGAGAATCAAGGGTAGGATTTAACCTGACACATTAGAC
ATTATCCCATGGCAACTCGCAACATGACTACTATCTTTGACTAAGTTATTTATTAAACTC
TTAAAGCTTTTATATTTATACCTGCAGTCCACAGTCACTACTATAATAAAGCAGAACCAT
ACCAGTACAGGTAGATTTAGCCAATACAAAATAGTACAGATCTAAATTATTGAAAGAGTG
GTTGTAAAATCTTTTAAAAACATAAAGACGCTATTGGCAGTTATACCTTGAACTCACAAA
TGTAATTGTATCACAAAGTTTCTAACATATTTCGAATAAAACTAAAAGGAGAGAAGACTT
CTTGAGCAAGAAACAAAATCATGTAAAATACACAAGCATAACATTGTAATGAGCAGCATT
GTTTTCATGTGGTTAATAATGATTAATATTAAAAGAAAACAAGCTAACTTCCCATAGCAG
GAAGCGTAGTAAGCCTAGCTAGTAGCTTTGCAACCAGCTGTAATAAAACGATTAATTATA
ATGACTTATAAATTTCATGTGAGTTTTTGGAGTTGATTAAAATTATTGACCTTAAGAAGT
AGCAGTTTAAATGGATTGTTTATAAACCATCAATGCAATTTTGCTTTACTGCATATGACC
AAAGCAGTATGGTTATTTTGAGTAGTCAACTAGCCATACACATGTGGAAGCCACACTCCT
ACACAGTTTATGTATGGTAGCACAATCCTACTTGTTGTTCAAACTAAAGATAGAAAATGA
TAAAAGTGGCTTATCAAATCCCCATAAAATTAGCACTGTTATAAATTTAAATAAATAGAA
AAGCCAAATAAATAGAAAACCCAAAACTTACCTGAACCCAAAGGGATGAAACAACCGTGT
CCTGTAGAAAGGTTTTGTAGATAAATAGAAAAGTTAGAATGGAAATCAACCTTTGTCTTC
TTTCTTAGATATCCAGTTTGTATAAAATCGTAAAATAAAAATGCTTAGCTCAAATCTACA
ACAGAGAACATAACAGGATTAAAGTATATAAGCAGTCGACACATACAAGATACACGTAAC
ACAACTGGCTGGCTATTAGACTTATATTATAAATGAGTCACCTCAGAGTAGTTGACGCAT
TACAAAAACAAAACGTACTCAAGTATTCATTGTTAAGCACACGAATTCAACTGAAGTAAT
TAAATAATTATTTTAATAGATCACTCCTACTGTATATACTAATTGAGAGGGTCTATACTT
AACCAGTGTACGTCACTAATTGTTTAGCCAAAGTTGATTGGAATATAAACACTACTTAGA
TATTTATATACGCACACAAGTTACCTCGATATAGAAACAAATTGTCATTTATTAATTTGA
TATTGTGAAAAATCAACCATAGTTTTTTTTAACAAGAACCTTTTTCTAAAATGGTAACAT
GATATAATATATACATACTGCAAAGCAGTTTTATTCATATATTAAGTGGTTTTTTACATA
ATTATAGTATTACTAATTTTGGAAAACAAATTATTTTACAGGCTGTTAGTTGCCCTGATT
AATCTATACAGAAAAATTTTGAAATGGTCCTTCAAGCAAATAATCAACATTTTGGTGGCA
TACTGACATGATAAGGGTTAATGAATTATTGCATCCAACACATGGGTTATATATGCCATA
AAATCCAACAATAAAAGGACAGGATTCAGTTCTATTGCCTTTATCAAGAGTCATAAACTG
ACAACAACAACAAGGCAATATTCGTAAATTTATTTTACATACGTCACATAATAAATTGCT
TTATGAACCCAAATTATTTAATAATACCAATATCTATAAGCTATGACTATACAAAGGCAA
TCATCCTTTTAAGGACTGATGTTGCAACCAACATACCTTGAGTATTGCAATTCTCTGTTT
TTATATTATACACAAAATCATAATACATTATTATATAGCATGACAAACCAGACTTCATAT
GACAAAACCAAAGCAATTTAGACTAAATGTGACGGGTTTTCTAAAAATGGGCTGACTGGC
TAAATGTACGGCATAAGAGCAGAGATTTAGGTCACAGTTGATCCATTATCCAAACACTAA
AAAAGTATATATCGGCTATCGCCCTGATTGGAAGTAATCGCCAGTTACGGCTTTCCAAGC
ACAGGTCGTTTGTAATCAATACTGCTGTATTAAATTTAATAATTAAAGAAAAGATCGGGA
CCGCTCAAAAATTCTTTTTAAATCTTGACAAAAAATATCCTCATCGCAAACACAATATTT
TCAACTCGGTTTGAGGCAAGCAAAAAATAAATATTCACTCTATATATATATATATATATT
TGCTGTTGAAGGTACCATTTTGTTTTTAATCTTTTTTCAATTGTTGTTTTTGTAGCCATT
ACTACCTGAATCAACAAATACTCCACAATTATTTTAATAGAACAAAAGTGTACAAGAGAG
CATACCAGCTCTTGAAACATAAGGTAGTTAATACAAAAGCAAAACCAAACTGGTTAAATC
TAAGTGCTATTAAAGTTTTACCTTTGCAAACTACATAGTAAAATACTTCCATAGTCAATT
TGAAGGCCGTATATTGTTGCACACCTTTTAACTTTAAAAATTACCTTATAACACTTTTAC
ACTCACTTTATAACACTTCAACTGATGAATACAACGACCTTGCAGTGGCTACTATATGAT
GGGAAAACAAATACAAACGCTCAGTTCTCATACATCGGAAATCATTTCACAGAACCAACA
AATAAGCGCACTACAGGGAGGACCTAGATTCTTATTCTTAGCACGGAACTTAGTAATATT
GTTTACTAACGTGTTCACATTTTAAGCACACTCAATTACACCGTACATAGCTGAAAACCT
AATATTACAACGGCAATGTACACCACTCGCAAACCGAATACGCCCAACTATCGATCCACT
CCGCTTCTGCGCGTTTCTTCTTTCGTTCTTGATGACCTCATTTCAAAAATCGATTTTTTT
TTTTCTTTTTTTTCGCGCAATCCGCGATAAGCAATTGGGGTATAAGTGGACTTGCAACAA
AAACAAATTTAGTTAATATAAATGTGTTAACCTAGTTTTCTCTACAATGTATGTATTTTT
TTAAAGTAAAATATTGTTAAGAATAATTTCAAAAATAGCGGGTACCAGAAAGCAACCAGC
AAAAATGACGTCACTGAAAAATGAGAGGTCGTTTCTGATTGGTTAAAATGAGTTCAAATT
TTACGATGTTTTTGCATCGTTTGACCGAAGGGATAAATGTAACCTCTTTGCCACTGTTTT
TCTCAAACGTATATGAGTCATCGGTGTTTAGTCAGTAATATTTAATAAAGGGATTCTTCC
CACAATAAACCCAAAACAAGTTTTACCAACCTAATTATATGCGTTGTGGTTTATGATACA
ATTACAAAATAGTTCTTTCTCACATGACGCAGAAACCCTGTTAGCCTGTTTATTACAAGC
AGTTGATTTCACGAACCATGTTTCTGCAACCAATTTAAAAATGAATTTTTTTTAAAGAGT
TTGTAACATTGCAACCTAAAGAAATGTGAAACAATACACAAACACAACATTGCTTGAGGG
TATAAATGGCAATTAAGATCTAATCAATCAACGCCTAACTAAACCACAGAAATTCCTTTA
TAAATATTGTTAATAGTTGATATTTAAGCGAAATCCTATTTAAAGCTGTATAGAGTTCTA
ACCAAAATAACACCTACTTTTGTGATATCTTGTATTTCCTTTGTAAATTAAATTGAAATA
TGGAAAACGTTTTAAATAAAAACACAATTTCATATTGCACTATAAATAGGTTTAAAATTA
TATAAGCAATATACTGCAAAAAATCGATGAAAGAAAGAAATTTAATATGTTTTACAAACT
GTTCATTTTAGAACACTGGGGCAAAAATACTATAATCAACATAAATGTCTGCAATATTAT
TTAATATTTAACTTTATGCCTAGCTTGTTAACAAAGATCCAGATCGTCTGATTGGCTAAA
ACTGGTC