Login
Help

CIS-REGULATION

Submit your Data

  1. Region 'REG00000160'

Cis-regulatory Region

Name

Ci-NPP -1098/-41

Short name

n/a

Region ID

REG00000160

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Brad Davidson (2007-04-19)

Annotator

Jonathan Luchino (2007-05-15)

Curator

Delphine Dauga (2010-04-07)

View position in WashU

Description

This sequence drives the entire Ci-NPP expression. Bioinformatics methods were used to identify putative Tinman- and GATA-bindings sites within the defined Ci-NPP enhancer. Future study will determine whether these sites are essential for expression of Ci-NPP.

Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.

Coordinates in the paper : -1100bp/-50bp

Hierarchy of Regulatory Regions

Regulated gene(s)

KH2012:KH.C2.514 (ENPP1; ENPP2; ENPP3)

Construct(s) made to test this region

CONS00000150: pCi-NPP -1098/-41 pfkh::LacZ

Show in situ data
Overview of functional TF binding sites in this region

3,308,4663,309,522

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [3,308,466 / 3,309,522] on scaffold KhC2

This sequence was found using the primers given in the article: NPPF1073-CGGATCTCCGATTAATG, NPPB36-GTCGCAATGCATGAG

Show sequence

Length: 1,057

CGGATCTCCGATTAATGATAGTCAAAAAATAATGTTACAGAATTATTCGACAGTATATTA
TCACGACTTTATAAAACGCGCGTCAAAGCCGATTACTGTTATTAAATATTTAAAAAAATG
GAAAAAAGAATAAGTATTTTTTGAACACGTAAAAATATGCGAAATAGTACGGTTCTAATT
TTGAATGCTCGCTAAATCATAGTGAAGACGATGAAATGTTGTAGTTTGCGAGTGTGAACG
ATTAAACTATAGTTTCATAGATTCTTAAATATATATATATCTATATGTGTATAGTTTTAT
TTAGTAAAAAAATGAGATTTAAACACGAGGAAATAGACAAGGGTCAATATGACTTTCATT
TGGGATAAAAACAACACAAAAATATCGTTTTAAAGTTATACATATATATATAGTGTTAAA
GGGAAATAAAACTAGCACAGATGGTTAATTTCTTCTCGTGTGTTTTAATATATATTTTTT
GAAATTGCTGTTGTCAATTAATAAAGGAACGAAACTACTAATTCGTGGTATCACCCAAAA
ACCGTCATGTATTTTGAATTTGGAAATATTGCGCAATATGTGCTTAAGTGTCCCATCTTT
CCCCACCCTACTGTAGTATGGTGGCGGTGGAAGGTGTCACACCTTTTTATTCTATTTTTC
TCGTCCCATTTGGTTGTAAACAAAGAACATTCAAAGAGTTATAAAACCGTATCCTCACGA
CTCCGATGGCCGTTGTTAATCTTTTAAAACACAATCAGAATATTTGGATATTAAGTGCTA
AAGGTGTCCCGTCTCCCCCCACCCTACTATATATATTATGAGCAGTCAATATTCTCACAG
AACGATATTAGATAGTGAAGAGGAAAATCGTACCCTGGAATTACATACAGTAACAAAGCT
ATGAGTTAGAAATCCCTTAATAACTATGAGCCTGGTGTTGTTATATAAAGTAGGACAAGT
ATTGTGACCTTTGTGCCGAGTAAACAAAACTGTTACCTAATCTACACCAGAGGCAACCCT
AATCCAGATCTGTTTTGTTTTTCTCATGCATTGCGAC

Reference(s)

Davidson et al. (2003). Evolutionary origins of the vertebrate heart: Specification of the cardiac lineage in Ciona intestinalis. Proc. Natl. Acad. Sci. U.S.A. 100(20):11469-73

Back to Top