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CIS-REGULATION

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  1. Region 'REG00000159'

Cis-regulatory Region

Name

29h10 -903/-350

Short name

n/a

Region ID

REG00000159

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Brad Davidson (2007-04-19)

Annotator

Jonathan Luchino (2007-04-19)

Curator

Delphine Dauga (2010-04-07)


Description

This sequence is the minimal enhancer which drives the entire 29h10 expression in endoderm and TVC, plus some ectopic tail muscle expression.

Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.

Coordinates in the paper : -900bp/-400bp

Construct(s) made to test this region

CONS00000151: p29h10 -903/-350::LacZ

Overview of functional TF binding sites in this region

203,988204,540

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [203,988 / 204,540] on scaffold KhL116

This sequence was found scanning the JGI v1.0 assembly with the published primers: 29h10F910-CCTACTGTATTCCATGC,
29h10B376-TTCAACCCCAAACGCATCC

Show sequence

Length: 553

CCTACTGTATTCCATGCACACCTCAAAACTATTCAATATCGCGTGATATATAGTTGCGTT
AAAAGGTTTACAAAATGTGACAGACAATTTATTTCTATCAATGCAAGTCAGCACCCTAGA
TCATCGCTTCTCTAGTCGCTGTAAATGGACTAAGTCAACACAGGGCTTTTCACTGTTTAA
AATTCCCGCGCCTGTATTTGTGGGGCGCGAACTAGGTGAGAAGATCATGTAAGAACAAAC
TGCGAAATTCTATCTCACGTTGGGGTATATAACTCAACAACTGCTGCAAAAATGTTATTT
TAAGGTCTGTATAAGTAAGCGAAGGCTTACCAATTAAGCACCCAAGCGTATAGTAGGTGG
TCTCTTGGATTCTCAGTGGCAATTCGGGACACATGAGTAATTAAATGATGCAAAAAATAT
TATTGTAAAGGGTGCAATAATGGATTTTTTAGTTGGTTACAGATTGACTCGTTGCACGTT
TTGAAAAGCCAAATGCGCCGCTCTCGGAACTGTCAGAGTAAATTAGACTAAAACGGATGC
GTTTGGGGTTGAA