- Region 'REG00000156'
Cis-regulatory Region
Name
Ci-tubulin-beta2 -4135/200 M2 mutated
Short name
n/a
Region ID
REG00000156
Status
Curated
Origin
engineered region from C. robusta formely Ciona int. type A
Type of Activity
regulatory_region
(extended_promoter)
Author
Kusakabe team (2007-01-28)
Annotator
Jonathan Luchino (2007-03-25)
Curator
Delphine Dauga (2010-04-08)
The authors look at the effect of a mutation in the M2 site on neuron-specific expression of the Ci-tubulin-beta2 genes:
The M2 motif is not necessary for the expression in the nervous cells.
Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.
Coordinates in the paper : -4300bp/-1bp
CONS00000147: pCi-tubulin-beta2 -4135bp/200bp M2 mutated::EGFP
156,051160,385
| Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment |
|---|
Aniseed Coordinates: [156,051 / 160,385] on scaffold KhL116
This Ci-tubulin-beta2 cis-regulatory region was found with blast on the JGI site with the Ci-tubulin-beta2 sequence. The ORF is reversed. The motif M2 had been mutated. Nucleotides mutated was replaced by "-".
Show sequence
Length: 4,335
GGCCAGCTTGCAGATGAACAATTTCTCGCATGATGAATAGAAACTAAAGATTGTTAAAAA
TATATAAAAATATTCTCTGTACTCGACTCAATCGCAGAATGACGACAGCACCACACGTCG
CGATATTTAAGCGCGCCTCGGCGCTCGATAT-------------ATGTAGGAAACTTTTT
TGTCGCGCGAACGCGGCGAATAATCAATTAAACAAAGAACGAGAGGGTCTCGTTTCTCTC
GGCGGAGTGGTACGTTTCCACGGGCTTCGAAAATCAACAGCCGCGGCTGCCTAGCAACAA
GACAATGGTGTCTGTTGACTCATTATAGCAAGCGAGCCTCAACTCGCCGTCAAACCCTTC
AATTGTAGAATACACCAGGTAAGGTGACCAGTATATGTCCCACAATAGTTACTATAGGGA
CGCCTTACATTTCAAACAACGCTATTATTTGATTCCTGATCGCGTCAAAGCGCGAGTCTG
TTGCCCGTGCGCGCGAGTTTTCCGCACGATTAGACAATGTTATGTTGTAATTCCCGAAGC
ATGCCGTAATCAGTGTAACCTAAATATCTGTAGGGCGTAAGATCGCAAACAAATGTGTCA
ACGAAAAAGACACCAAAAAATATTTATTTCGAATCCGTACAGTAACACATAACACTGAAT
ATCATAGTCGGTCGTCATCCCCGTGTGTTGCAATGAAATCCTGTGTACCGCAATGCAAAT
AATGACAAATTGGATTATTCATTACGTTTAACGTTAGCTTATGTTGGACTTTGATTTATT
TGACTGTATTTGTGTTATGCTTTTGGCTTTTTAATTTTCGTGAGTTATTTAACTTTGTGT
AGCTATTATGAACCCCCCATGCCCACGCTCAAGTTACTGTGTTGCAGTATATTGATTGTT
GGTGTGTTTTTTATAGATTTGTTCTTAAACTTAAATACTTATATGTGGGAACTTTACTAT
CTAAGGCTAGCTTGTGGCCATATTCAAATTGTACAATTGATTAACAAGCACATTTTCTTG
AGAGGTTTCAATGTTCATTAATTGTCCTTCTAAATACAAACAATCTCTTTAGTGAGCCCA
CTAAGTATAAAAAGCTACAACAATCCCAGACGAAACAGTACACTTGGGAGTAACTAGCAT
TTTAATCTTCACCATTCTAGCCTATCAAGTGTGCTTGATTTCAATTTATCGACAAATGAA
CTCCTGCGTGGATACGCCGAGTGTGCCGGTATTGATGTTTTTATAACTTAACAATCAATA
ATTTTAGCGTGCTATTAAATTGTTACGAAAGTGCCATTAATAATAATGCAGTTTGTCGAT
TTCCATACGGTAAAAACTCCAAATGTTCTTAATTTAACCAAGGCTAGACAAATTTATTTG
TTTTCTTGCAGTGAGCGGGCCATATGTTAGTGTATAAACTGCACGCTGTTAAAAAAGAAT
AGCCCTATTTTAGAATCCAGTGCATTGTTATGCAACAGTTGGTGTTCGTTGCGGCAAGAC
AGCATCACAGAATAATGAATTCCCGTTGGGAGGACAGGTGCAGGGTGTCTGTATGACGTC
ATAAGGGAGACTGTGAGTAATTTATTTGAGGGTTCGATATATTGGACGGCATCCAATCAC
GTAGCGCGAACTTATCAATAATTTAGAGGCTGAAACGGTGGGGTTTGAGCTTCAACGAGA
ACTCTTCCTTTGTATCATCACAAATAATGTAGGTTAATCTAAACACCGAGAATTCGGCTG
TTATTTTCCTTATACAACACAATATAAAACTCCAACCCCGCGCTTTGTTTTGATTTAAGG
TCAATTTTGTACGTCAAAACCGTAAAACTTCGATAGTTTGATTATACGTATTACTTGACT
TAGTGTATGGATGCTTTCATTTTTGCTGTTGTTTATCTGAACAAATACAACGTACACATG
AAACCTTAAACAAATGTGAAAATAAAGTGGATTGAGGAAGATAAAACGCATCTGCTGTTT
CCTGACCTTTGTTGCAGCATACACTGCGTGAAAAGGTAATCATTTTTACTATAAAGCCTG
AACCAAAATGTCAGAACTTATGGTAACTTAATACAATTCCGTGAAAGCGATAGAAGCGCT
TTGTTATCGAATTCCAATCTCGGCTTGGAAATTGATATTTGTACAAGACGCGACGCTAGC
GACGTGCCCGATCAAGTCAGGAATAACATTCCGTTGCTTGGTGATGAAAAGAAGTACCTT
CATTCCTTCGAATTTTGTCTTCGTGGGTGAGACTACCAACCGAACGACAATGTATTGTCT
TAATCGCACAACAGCTGCTATAGTTCTTTTTAACAATGGAATTACGCAATTAAATTTAAT
TCTAGATTAACGGATTAATTAGTTGGAAAACTCTCATTTACGGTTACTGATGTTCGTTTT
CTACCATGAACTACATAGCTGCATCGCTAATTAAAACCATTGTCCAAAATTATTGAGATT
TAAACGCTATTGGTGCAGTCAGTCAGTGATTTGTAAAACCGATAAAAAGTGACGGGAAAA
CTGAACTGCTGGCAATAGTAAGTACAATAAAACGCAATCACGCTTTAATAAAACTAGAAA
AAGTAGTATGGTATTGGAAGTACGTTGCTATTGGAATAATGCACAGTTAATATAGTAGTG
TGGGGTGAGATAACACTGTTTGCACTTAAATCCCATATTTCCTGAACCTTTTTTGCACAG
TTAACAATTCTCCTTTAAAGTCGCGGCCTATAATTGTTACAGTAGAGTGGGGGAGGCCGC
GACACCTTTAGCATATAATATCCAAATATCCTGATCGTGTTTTAAACAAATAACAACGTT
CTATGCTGGAAGTCGTGAGGATACGGTTCTATAATTCTTTGAATGTTCTTTGTTTGCTAC
CAGATGGGACAAGAAAGAAAAATAAAAACATATTCCATCGTATCCAACCCTTTTATAGTA
TTAAAAAATCACTTGCCTAATAAATGTGATGATGTCATAATTTTGGTGTTAGACACATGT
AGCTGTTTGTATACAGTCGCTTGGTACTTGTACAACTTTACGGCGTCGTCTTATATTCCC
TAATGCATAATTCTCATCCATACCTGAGTCACGCGCTCGCTGCATTTGGCCTAGCATAAA
TTATTCATGGTCAATCGATTTGAGAATGCATCCCGGTTTTGCCGGTACTTCCCATTACAA
AGTACAGGTCGATCGATAGAAAACCGCGCCCAAGACCGAGTCTTGGTGTCTGTGTCTCAG
TGTTACGTCACACAGACGTGATTCGACATTTTGGTCCTGAGCGTGTGCAGAACAGTCGTC
GCATGAACAATTTTCAATCAAAACTGCGAAAATATTAAAAGTTTTATTACTATCAAAATA
GTTCTGTTTCTTTTAAATTTAAAATTAAACCCTAAATCAAACAGTTAATTATTTAGCACT
TTATTTTGCTTTTAAGAGCTTCTTTTAAAGCTTTAAGGTGCTTAAAATTTTGATCGTTAA
AGATTCTATTCTTAGTTTATCAATAACATTCTTACCGTCCAACCGTAAGCAGACTAGCAG
TTAATTTTAAAAACAGTCGTTTTGGCTTCGCCAGCTCTGCAACGCAACCTAACGGATAAA
AATCGAAATACGCCATAACAGCTTTGTGGCACACCTATCAACGAATAAGATTTATTGCAT
CCACGCAACGAAGTCTGTACAGGCTTCTTGTTTCAGACACCTTGTTTCGGCTAACAAGTA
ACCACATTTTTGACTTTGCTGGTGAAGTAATAACATGTGATAATTTTTAATTTATTTTGT
TTTGTATGGCTGAAAATTTGGACAACCTATTAGTGATCTAGTAGTTGGATTAATGTTGTT
TTGCACACATATTCGGTATCAGTGTCGAGTCTCAAACTAGCAACCTTTGGTCCAATGCGA
GTGCCTAACCACTAAGCTATGGACAATTCCTTCACAAGAACAGTAACTTCCAACTTTAAA
TTCATTATCAGTTTATTAAACAACATAAAAACACTAGTACTACAAAAACAGTGAGCCAAG
GTATTAAATTCATTTTTATGAATGGTAAGGCAACAGATATCAAATTATTACAAAAATTGT
AAACAAACTGAACTTTCACAAACAGCCAATAGCAGCACTGTAAGTCTGAGTTATTACAAA
ATTATCAGTTGTTTTACCGGTTTTAACAAAAGTTTTGAAATGATTTGCATAATGCGGAGT
TCGCTAGTTTTGCAAATTAAAGTATAAAAATAACATTTAAAATACAGCATGTTATACTTT
CAAAAGTTCTACATACAATTGTAAAATTTAAACTCTGTTTAATTTCGTAACAATAAGCAC
CCTCAGTGCTTGATC
