Region from -553 bp to the +39 bp is the minimal enhancer which reproduces the spatial and temporal expression pattern of the endogenous gene Cititf1.


This region includes three distinct regions of regulation.

- a central region, involved in activation of the expression in the endoderm, is situated between -481 and -328 bp of the transcription start and contains cis-regulatory sequences associated with the endoderm-specific transcriptional activation.

- another region of control, required to achieve an efficient gene expression, lies between -553 and -581 bp.

- finally, the enhancer proximal region includes sequences relevant for the activation of expression in the mesenchyme cells, expression that is normally silenced by negative control elements between -481 and -433 bp.

Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.

Coordinates of this region in the paper : -355bp/+238bp

Name of the construct in the paper : construct M20

Modification of Cititf1 -553/39 by substitution

KH2012:KH.C10.338 (NKX2-1; NKX2-2; NKX2-4)

CONS00000129: pTitf1 -553/39 M20 mutated::LacZ

3,636,4203,637,011

Aniseed Coordinates: [3,636,420 / 3,637,011] on scaffold KhC10

This sequence was found using a blast on the JGI site with the sequence includes in the publication listed bellow. The nucleotides in position -205bp to -201bp are mutated by transversion.

Fanelli et al. (2003). Interplay of negative and positive signals controls endoderm-specific expression of the ascidian Cititf1 gene promoter. Dev. Biol. 263(1):12-23