- Region 'REG00000090'
Cis-regulatory Region
Name
Ci-Mesp -107/39
Short name
n/a
Region ID
REG00000090
Status
Curated
Origin
natural region from C. robusta formely Ciona int. type A
Type of Activity
inactive_region
Author
Brad Davidson (2007-03-11)
Annotator
Jonathan Luchino (2007-03-11)
Curator
Delphine Dauga (2010-02-26)
Characterisation of a minimal cardiac enhancer for the ciona Mesp gene demonstrated direct activation by the T-box transcription factor Tbx6c : The T-box domain to -110 bp from -102 bp upstream the +1 of transcritption of the Mesp gene is sufficient to drive reporter gene expression in the B7.5 lineage of electroporated embryo.
JGI transcript model was taken as reference for the region s coordinates (same in the paper).
Modification of Ci-Mesp -110/39 by deletion
- Ci-Mesp -150bp/39bp REG00000643 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -138/39 REG00000211 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -150bp/39bp Lhx3-1 mutated REG00000644 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -150bp/39bp Lhx3-2 mutated REG00000645 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -150bp/39bp Lhx3-3 mutated REG00000646 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -150bp/39bp Lhx3-4/5 mutated REG00000647 [ regulatory_region (extended_promoter) ]
- Ci-Mesp -150bp/39bp Lhx3-6 mutated REG00000648 [ regulatory_region (extended_promoter) ]
3,537,1593,537,303
| Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment | |
|---|---|---|---|---|---|
| 1 | T-Box | n/a | CGGCGATAA | [-82 / -74] | |
| 2 | T-Box | n/a | TAGACACCT | [-63 / -55] |
Aniseed Coordinates: [3,537,159 / 3,537,303] on scaffold KhC3
The sequence was retrieved from the JGI v1.0 assembly using the sequence found in the paper. Deletion of the first three bases of the parental construct.
Show sequence
Length: 145
CGATAATGACTTTGCCCGTTTCATGCGGCGATAAACGAACTAATTAGACACCTCCTACAG
ATATAATGGTAATTCAGAATCGTGTGGTTATGTAATTCACAAAAACATTTTAACAAAACA
GGTTGATTTGAAACTTGTATTATGG
