Description

Characterisation of a minimal cardiac enhancer for the ciona Mesp gene demonstrated direct activation by the T-box transcription factor Tbx6c.



JGI transcript model was taken as reference for the region s coordinates (same in the paper).

Hierarchy of Regulatory Regions

Modification of Ci-Mesp -138/39 by deletion

Regulated gene(s)

KH2012:KH.C3.100 (MESP2; MSGN1; PTF1A)

Construct(s) made to test this region

CONS00001546: p.Ci-Mesp -110/39>hCD4::mCherry

CONS00001547: p.Ci-Mesp -110/39>hCD4::GFP

CONS00001554: p.Ci-Mesp -110/39>RFP

CONS00001271: p.Ci-Mesp -110/39>H2B:mCherry

CONS00000079: pCi-Mesp -110/39 pfkh::LacZ

CONS00001501: Ci-Mesp -110/39>GFP

CONS00001503: Mesp>HA::nls::LacZ

CONS00001270: p.Ci-Mesp -110/39>H2B:GFP

CONS00000080: pCi-Mesp -110/39 pfkh::GFP

CONS00001272: p.Ci-Mesp -110/39>mCherry

CONS00001500: p.Ci-Mesp -110/39>nls:LacZ

Overview of functional TF binding sites in this region

3,537,1593,537,306

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment
1	T-Box	n/a	AGGCGATAA	[-110 / -102]
2	T-Box	n/a	CGGCGATAA	[-82 / -74]
3	T-Box	n/a	TAGACACCT	[-63 / -55]

Sequence

Aniseed Coordinates: [3,537,159 / 3,537,306] on scaffold KhC3

Information extracted from the publication "Uncoupling heart cell specification and migration in the simple chordate Ciona intestinalis" by Davidson B, Shi W, Levine M. Development. 2005 Nov;132(21):4811-8. The sequence was retrieved from the JGI v1.0 assembly. The proximal end of the clone was retermined using the reverse primer indicated in the article: Ci-MespNatb, aaaGCGGCCGCCATAATACAAGTTTCAAATCAACCTG

Reference(s)

Stolfi et al. (2014). Divergent mechanisms regulate conserved cardiopharyngeal development and gene expression in distantly related ascidians. Elife 3():e03728

Davidson et al. (2005). Uncoupling heart cell specification and migration in the simple chordate Ciona intestinalis. Development 132(21):4811-8

Razy-Krajka et al. (2018). An FGF-driven feed-forward circuit patterns the cardiopharyngeal mesoderm in space and time. Elife 7():

Davidson et al. (2006). FGF signaling delineates the cardiac progenitor field in the simple chordate, Ciona intestinalis. Genes Dev. 20(19):2728-38