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CIS-REGULATION

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  1. Region 'REG00000046'

Cis-regulatory Region

Name

Ci-MRLC2 -490/80 M2 mutated

Short name

n/a

Region ID

REG00000046

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Kusakabe team (2007-01-28)

Annotator

Clara Degos (2010-07-22)

Curator

Delphine Dauga (2010-05-20)

View position in WashU

Description

The M2 motifs are necessary for the expression in the muscle cells.

Hierarchy of Regulatory Regions

Modification of Ci-MRLC2 -490/80 by substitution

Regulated gene(s)

KH2012:KH.C8.309 (MYL2; MYL5; MYL7)

Construct(s) made to test this region

CONS00000028: pMRLC2 -490/80 M2 mutated::GFP

Show in situ data
Overview of functional TF binding sites in this region

4,042,9534,043,522

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [4,042,953 / 4,043,522] on scaffold KhC8

This sequence was found using blast on the JGI site with the sequence includes in the publication sited bellow. The motif M2 had been mutated (TGTGACGACAGCA -> CAGAGTCGACATC).

Show sequence

Length: 570

GCAGCAGCACAGCATATTGTAGCATTAATGTGATCAAAATTGGGGTAATGGGCCACGTCT
GGCCTAAATCCCAGGTCCTAACGGACCTCACCCATACAGAATGTGATCAAAAAAGTTTCT
TAATGATCGTTAGTTAACAAAATCCATTTAGCAAGGTGATATTGCTGTTCGTATAAGATT
CTACATATTTTCAATTTCGTCATCGCAAAAGCAGTGTTGGATCGACGCGGGTTGCCTATA
GTACGATTTATAACACTATTTATCTTGACACGTTGCGCTGAACAATGTAGAGACTGATAT
ATGTTGCTTGAGTTTGCGCAACGTGACAAGCGCATCGCCCCTGCATATCAAGAACAGAGT
CGACATCGTGTTGTCACTTACATCGTTTGTGGGGTATAAAAGGCGAGGCCCGCCTGGTAA
GAAATCATTCCAACTATTTGACTCTTCAGCTTACTTGCTTCTTCAGCTAACCAGACAAGG
AATTCTTAAAATGGTAAGTTGTAACATTCTCATACCGATCAAGAGATAGAGTTTAAAACT
GACTTTGAATTCTTAGGCCGGTGGACGAAA

Reference(s)

Kusakabe et al. (2004). Computational discovery of DNA motifs associated with cell type-specific gene expression in Ciona. Dev. Biol. 276(2):563-80

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