Region from -658 bp upstream of the putative transcription start site is the minimal enhancer which drives the entire Ci-ZicLB expression (the transcription start site is the nucleotide +1).

The 259-bp 5 flanking sequence contained essential elements for driving a correct spatiotemporal expression. This enhancer can be divided in two distinct modules:

- The A module was located between nucleotide positions -259 and -205 upstream of the putative transcription start site, and was necessary for activation in A6.2 and A6.4 blastomeres at the 32-cell stage.

- The BM module lay between nucleotide positions -205 and -89 and was responsible for activation in B6.2 and B6.4 blastomeres at the 32-cell stage and in A-line presumptive notochord, nerve cord, and muscle lineage cells at later stages.

Two putative Fox-binding sites, one located within and the other downstream of the BM module, were necessary for the latter activity. Mutation at a potential Ets-binding site, located downstream of the BM module, caused ectopic
activation of the reporter gene in a-line presumptive ectoderm
cells.






Be careful with JGI V1.0 for this locus, the sequence is duplicated.

Modification of ZicL -205/codon7 by substitution

KH2012:KH.S816.1 (ZIC2; ZIC3; ZIC4)

CONS00000025: pZicL -205/codon 7 Fox4/5 mutated::LacZ

3,2713,588

Aniseed Coordinates: [3,271 / 3,588] on scaffold KhL59

The sequence was found in the publication like all the motifs sequences. The Fox4 site AGGCAAATATCG was mutated into AGGCGGGTATCG, The Fox5 site AAATAAATAAAG, was mutated into AAATGGGTAAAG.

Anno et al. (2006). Transcriptional regulation of ZicL in the Ciona intestinalis embryo. Dev. Genes Evol. 216(10):597-605