Region from -658 bp upstream of the putative transcription start site is the minimal enhancer which drives the entire Ci-ZicLB expression (the transcription start site is the nucleotide +1).

The 259-bp 5 flanking sequence contained essential elements for driving a correct spatiotemporal expression. This enhancer can be divided in two distinct modules:

- The A module was located between nucleotide positions -259 and -205 upstream of the putative transcription start site, and was necessary for activation in A6.2 and A6.4 blastomeres at the 32-cell stage.

- The BM module lay between nucleotide positions -205 and -89 and was responsible for activation in B6.2 and B6.4 blastomeres at the 32-cell stage and in A-line presumptive notochord, nerve cord, and muscle lineage cells at later stages.

Two putative Fox-binding sites, one located within and the other downstream of the BM module, were necessary for the latter activity. Mutation at a potential Ets-binding site, located downstream of the BM module, caused ectopic
activation of the reporter gene in a-line presumptive ectoderm
cells.






Be careful with JGI V1.0 for this locus, the sequence is duplicated.

Modification of ZicL -658/codon 7 by substitution

KH2012:KH.S816.1 (ZIC2; ZIC3; ZIC4)

CONS00000015: pZicL -658/codon 7 Ets4 mutated::LacZ

2,7903,588

Aniseed Coordinates: [2,790 / 3,588] on scaffold KhL59

The sequence was found in the publication.

Anno et al. (2006). Transcriptional regulation of ZicL in the Ciona intestinalis embryo. Dev. Genes Evol. 216(10):597-605