Aniseed - Cis-regulation

Cis-regulatory Region

Name

Ci-vAChTP -3791/-1

Short name

n/a

Region ID

REG00000031

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Kusakabe team (2007-01-24)

Annotator

Delphine Dauga (2010-01-15)

Curator

Delphine Dauga (2011-08-17)

View position in WashU

Description

Data from DBTGR (Sierro et al., 2005). Region from -3791 to -1 from the start codon allows expression in neurons of the brain vesicle, the visceral ganglion and the dorsocaudal epiderm. Reference for promoter is: Yoshida, R., Sakurai, D., Horie, T., Kawakami, I., Tsuda, M., Kusakabe, T. 2004. Identification of neuron-specific promoters in Ciona intestinalis. Genesis (39): 130-140.

Hierarchy of Regulatory Regions

Modification of Ci-vAChTP -4922/-1 by deletion

Ci-vAChTP -4922/106 REG00000900 [ regulatory_region (extended_promoter) ]
- Ci-vAChTP -4922/-1 REG00000518 [ regulatory_region (extended_promoter) ]
  - Ci-vAChTP -3791/-1 REG00000031 [ regulatory_region (extended_promoter) ]
- Ci-vAChTP -4660/106 REG00000867 [ regulatory_region (extended_promoter) ]

Regulated gene(s)

KH2012:KH.C1.498 (CHAT; CPT2; CRAT)

Construct(s) made to test this region

CONS00000211: pCi-vAChTP -3791/-1::EGFP

Show in situ data

Overview of functional TF binding sites in this region

4,530,8634,534,652

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [4,530,863 / 4,534,652] on scaffold KhC1

This sequence was found using a blast on the JGI V1.0.

Show sequence

Length: 3,790

TTTTAAGCACACGTTCAAAAATTTAATAAAAGTTACAATAAGTTATTTAGCGATTTGCAC
AACAGCTGAAATTTTACCAATTCAAAATCACGCGGGGTAAGATGAGACTGTTTAAAATCT
TTATTAGTTTTGAATAATAAATGAATAATATTAGAAATACATGTAAACAACCCGAAATAA
TATTGTACGCTTTGTAAATGAAAATTAAACATTCTTTCCTTGATCCCTGTTATAGTTTTT
AACACGTTGCATCCCGGTTAGCAAATGTTCCACCTTACCCCAGTCCCGTCTTCCCCCTAC
CTACTATCACTCTTACGATTTTCACATCCCCATGTGACGTATAACCACATAGGCATAGTT
CCGATCGAGCGATGAAACAGTGATGTTGGTTAGCGTGGGGTAAGATGAGACACTTAAGAA
TATGTTTTCTAATATTAAAAAAATAATAAAATTGACTGTTTTTTGGTCATGGCATGAGTT
TGTATTATCATAGCTTTATTAACTGACAACATGAAATATATAAACCACATATAACGTATT
TATGGGAATTGATATTGTTTGAACGATAATTAATAAAAATTGTATATCCTACAGATGCAA
ATAAACTTATAACAACAGAAAGTTGACAATACTTAATAATGAGATTATATCGGTATATTA
AGTTTAAACTACTCTTGAGGTTTAAATTATTAAATAACAAACAAACCGTTTAAATTTTTG
GGTTTGTTTTCTTTTATTGTTATCCTATCCTTATGGGTCAACCCTCTTTAAAGCTCCTTT
TGTACGGGTCGCGTCTTTACGTACATTTCTGTCTCTATTAGAAGTTTAACTGAAAATAAT
TAAACATAAATGACTCGTTTGCATGCTAGGTGTAGCGAACACGCTTATTTTCTTCCAATT
ATTGTAAATATGTTCTTAAATGTAAGCGTATTTTAACAACTGTTGTTTTATCGCAATATC
CTGCATTTTTGTTTTAAATACTTTTAAGTCTTTGCTACTATAATCGAAAAGACAAATAAA
GTAATTATTAAAAGGTTAACAGGTTTTATGTCAATAATATTCTGCAAAACAGTGTTTTTA
ATTTTATTTTTAATCCAATTCATACTGCTGGCTCCTGCCAATTAATAAAGGTACATTGGC
GTGGGAAAAGATGGGACACCTTTAGCACATAATATCTAGATATCCTGATCGCATTTTAAA
CAATTAAACGGTCTAGGAGAGTCGTGAGGGCACCACTTTATGATTTTTTGAATGTTCTTT
GTTTACTACCAAATGGAACTATAGAAAGTCGAAGAAAAAGGTGTCCTACCTTACCCCACC
CTACGATATATATCCGTATTTCTTAAACGGATTTTATGAAACAAAATCAAACAGTACGAA
ATATTTCAGCCTATTTTATTTAACTAAAGTACTTGATTAATGATTTAAACTAGCAAACTA
CAAAATTTTGCCAATTTTAATATGATTTACCGAGCAACTTTTATTACTTAAAAAAACGGT
AAATGCCTTTGCCGCGCAATTTGTTAGACTGTGAGAATCCTGTTGAATGTTACTAACATT
TTGTTTCACCTCTTATCAAATAGGTATCCTACCCAGAAAAGAAGATTTTACAATATTCTA
TTTTACCCCATGTTCCACGATTCCCCACTCTGCTATATAATAGTATTTTAAACAAGTCGT
AGTTTATAGAGGACTTGAGCGAAACTTTTATTACGTCGTAAACCTTTGGCTACCATCATC
TGCCTCAAAACAAAATTAATTAAAGAAATGCGTTAGTGTATCCTTTGACTCGGAATCAAT
CAAATCAAGCAAAAATCAATATGTGAAATTAACCATTTAGACCTTGTGTCATTCCCATTG
CGGTGACTTGTCCTAGTTGTGCGTTTTTATCAGCAGATTGATTTAACAGTCACCGGAACA
GGCAAGACAATATTTCAAACCAGCCAATGTTAATTTCAGACAAATGAAGCAATCTGAAAA
TCAGAACAAATCAAAAAACATAAGTTTTGGTTTTTAAAGCATAGAAAACGTACCGTATTT
TTTAATGTAATTGTTAAATTTTGTTATTTAATATAGTAGGGTAGGGGGAGATGGGACACT
TTTTCATTCTGTTTTCTCGTCTTGGTAGCAAACAAAAACATTAAAAGAATTATAAAACCG
TATCCTCGCGACTCCTACAGACCGTTTAAAACAGGATATTTGGATATTCTGTTCTAAAGG
TGTCCCATCTTACCCCACAGTACTATACACTATACACATTCTGTACCGATATATTTTATT
GATTAAATTTGAAGTTGTTAAACTTAATTACGATTAAATTTCGGCAAATTGAAAATGAGC
CATGAATTAATCAAAAATTATTCTTGATCGTTGTTTTGTAAAACATAACTTTTTTTTGAT
TTTTTTGGGAGGCGCCCTGTGTTCCACTTATTATTATTTGTTTGTTTTGCTATGTCAATA
CTTTAATATAAAAGAAATTAATATTGGCTGACATTTCAATTTAACGCTAGGCTTATGTTT
TGTTTCGTGAATAATCTGCATAAAGAAAAAAAGCAGTATCGACTCTCCTATTGTTGAATC
ACTCTTGCTTCCCTTCCATTGTGCAGGAGATAGTGACGCAATGAACATTGATTTTAACGC
TTCTAGTCAGTTGGGCCCTTGCTCACAATTGCCTAAAATTTGATCAATTGTGGATTGAAA
AGTTAATATTCTTTCCTGAATTAACATGTCTATAGGTAAGGTATTGAGACGGCTCCCATT
TAATTTCTTGTCCGATCTTTATAAAAAAATATTTGTTAAAGGTACGTTTAATTTGAACAT
ATAGTGCAGAAGTCTTCGGAATTTTCATATAGAGTTATTTTTTAGATACTATATGCGACA
TATTTTCATAGCCCAAATTAAATTGTTTTATAATTTGTTTGGCTTTTACAGATTTTAATT
TAACTTAGGTGTACGATTCAATAGAAAGATTTTAACCTGTAAAAATACGTGCAAGTTGTT
TGGAAATACCTTTTTTGTTGGACAAAGTTGTATCAGGTAAGTTGATAGCATGTATCTTAA
ATCTGGCGTGGTGTTTATGTATTGGCTTCATCATGAAATAGTTTGTTTTGTCCTTTTACT
TGTCAATTTTATTTAAATTACATGAGGGTACAATTCATTATAACGTACTTCGGTGAAAGG
TTAATGTTAACAAATGCCGTCCGGTCTTCTGCGTATCTTTCGATTTCGTATTAATTATGC
ATAGAAAGGGTTTAAGTGCAATGCTATTTCTGATGATGTGTGTGTAACACCAACGACCTG
ATGACGAAAAACTTGACTGTTTATTAAATAAAATAGCGCAACAAGACGGCGGAATTTATA
AATGGCATGTTGCTCGTAAACAGCAGCGCGTGCCTGTTTTAACTCTGGAGAAACAAGTTA
TATTCAACTATTTGTTATCTCACAAAGCACGGTATTGCACTCTTATCTAATGTACTAATA
CAAATATATATATATATATATATATATATATCTAGCTGTTGTCGAGGTAAGATGAACTGC
AGTTAACAATAAAAACCAACTTCGTTATACACAAATTCAATATAAAGCGACAGCTCAGTG
TTGTAGAGTAGGTACCCAACTTTCTTAATCTGCAAAAGGCAAATACATGATTTATAATAT
GTAACTCAGCATAACAACCTGTTTTTGCCTTTTGCAGATTACATCTGAGGGCGGGTTGTG
TGGGTCAGAAATTTATCAAAGGAAAATAAAGTTGATTGAAAGCAAATTTGTTTCTACTTT
ATTGTTCATC

Reference(s)

Yoshida et al. (2004). Identification of neuron-specific promoters in Ciona intestinalis. Genesis 39(2):130-40

Sierro et al. (2006). DBTGR: a database of tunicate promoters and their regulatory elements. Nucleic Acids Res. 34(Database issue):D552-5