1 result
OVERVIEW
Click here to see the RNA-Seq Pipeline Protocol.
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Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Halocynthia roretzi (Aomori, Japan) were fertilized by sperms of an another single individual and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the AGPC method and the quality was checked with the Bioanalyzer, most samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
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| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 15 (mid neurula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
3 results
MAGEST annotation : a4.2, b4.2, A4.1, B4.1.
| Stained molecule | Harore.CG.MTP2014.S2.g06158 (ARMC1) |
| Stained region(s) | A4.1 cell pair - a4.2 cell pair - B4.1 cell pair - b4.2 cell pair - |
MAGEST annotation : epidermis, brain, nerve cord, A-line notochord, B-line notochord, muscle, mesenchyme, trunk lateral cell, trunk ventral cell, endoderm.
| Stained molecule | Harore.CG.MTP2014.S2.g06158 (ARMC1) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - A7.6 cell pair - A8.13 cell pair - A8.14 cell pair - A8.15 cell pair - A8.16 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - a8.21 cell pair - a8.22 cell pair - a8.23 cell pair - a8.24 cell pair - a8.25 cell pair - a8.26 cell pair - a8.27 cell pair - a8.28 cell pair - a8.29 cell pair - a8.30 cell pair - a8.31 cell pair - a8.32 cell pair - A8.5 cell pair - A8.6 cell pair - A8.7 cell pair - A8.8 cell pair - B7.1 cell pair - B7.2 cell pair - B7.5 cell pair - B7.6 cell pair - B7.7 cell pair - B8.15 cell pair - B8.16 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair - b8.21 cell pair - b8.22 cell pair - b8.23 cell pair - b8.24 cell pair - b8.25 cell pair - b8.26 cell pair - b8.27 cell pair - b8.28 cell pair - b8.29 cell pair - b8.30 cell pair - b8.31 cell pair - b8.32 cell pair - B8.5 cell pair - B8.6 cell pair - B8.7 cell pair - B8.8 cell pair - |
MAGEST annotation : epidermis, adhesive organ, brain, nerve cord, mesenchyme.
| Stained molecule | Harore.CG.MTP2014.S2.g06158 (ARMC1) |
| Stained region(s) | anterior sensory vesicle - epidermis - Palps - posterior sensory vesicle - tail nerve cord - |
